Faculty Directory

Dr. Subhasree Roy Choudhury

Scientist-C

Development of target specific nanotherapeutics for epigenetic regulation of cancer. Chemoprevention and mechanism of action of drug/siRNA loaded nanoparticle mediated therapy for leukemia, neuroblastoma and glioblastoma. New drug development from natural products and combination therapy against cancer. Development and application of high-throughput functional gene-based screening for cancer. Multiple retrovirus and lentivirus mediated gene delivery for target validation and functional implication in cancer.

CONTACT INFORMATION :

Research Interest

  • Development of target specific nanotherapeutics for epigenetic regulation of cancer. Chemoprevention and mechanism of action of drug/siRNA loaded nanoparticle mediated therapy for leukemia, neuroblastoma and glioblastoma. New drug development from natural products and combination therapy against cancer. Development and application of high-throughput functional gene-based screening for cancer. Multiple retrovirus and lentivirus mediated gene delivery for target validation and functional implication in cancer.

Research Highlights

  • Dr. Subhasree Roy Choudhury and group have recently shown that Polycomb protein Bmi1 is associated with advanced prognosis of acute myeloid leukemia (AML). Polyethylenimine (PEI)-stabilized Bmi1 siRNA-entrapped human serum albumin (HSA) nanocarriers (PEI@HSANCs) were used to protect siRNA from degradation and for epigenetic regulation-based AML therapy. Nanoform increased the transfection efficiency of Bmi1 siRNA through caveolae-mediated endocytosis and enhanced Bax translocation into the mitochondria, enhanced caspase 3-mediated apoptosisand inhibited Bcl2. The molecular analysis reveals the downregulation of polycomb proteins, Bmi1 and EzH2, along with inhibition of H3K27me3 and H2AK119ub1 through ubiquitin-mediated degradation of Bmi1, which is reversed by a proteasome inhibitor. Further mechanistic studies by Chromatin immunoprecipitation (ChIP) assay established a crucial role of transcription factor, C-Myb and Bmi1, as its direct targets for maintenance and progression of AML. Decreased leukemic stem cells marker (CD45+) and an increase in the myeloid differentiating marker expression (CD11b+) in the bone marrow,withdrawal of epigenetic repression through ubiquitin proteasomal pathway potentiating a novel antileukemic therapy were established in in vivo AML xenograft model.

    More details: ACS Appl. Mater. Interfaces 2020, 12, 23, 25633–25644. https://doi.org/10.1021/acsami.0c06186

     

PhD Students

  • Ms. Babita Kaundal

    Email: babita.ph16209@inst.ac.in

    Reg. No.: Ph16209

    Working Since Jul, 2016
  • Mr. Mohammed Nadim

    Email: nadim.ph16240@inst.ac.in

    Reg. No.: PH16240

    Working Since Jan, 2017
  • Mr. Avinash Chandra Kushwaha

    Email: avinash.ph17210@inst.ac.in

    Reg. No.: PH17210

    Working Since Aug, 2017

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  1. Epigenetic Regulation of Bmi1 by Ubiquitination and Proteasomal Degradation Inhibit Bcl 2 in Acute Myeloid Leukemia: Avinash Chandra Kushwaha,Soni Jignesh Mohanbhai,Mohammed Nadim Sardoiwala,Ankur Sood,Surajit Karmakar* and,Subhasree Roy Choudhury*, (2020) ACS Applied Materials and Interfaces, 2020, 12, 23,: 25633–25644.. DOI: https://dx.doi.org/10.1021/acsami.0c06186

  2. Melatonin/Polydopamine Nanostructures for Collective Neuroprotection based Parkinson’s Disease Therapy.: Srivastava, AK, Roy Choudhury S*, Karmakar S*, (2020) Biomaterials Science, . DOI: https://doi.org/10.1039/C9BM01602C

  3. Recuperative Effect of Metformin loaded Polydopamine Nanoformulation promoting EZH2 mediated Proteasomal Degradation of phospho-α-Synuclein in Parkinson's disease model: Md Nadim Sardoiwala ,Anup K Srivastava ,Babita Kaundal ,Surajit Karmakar ,Subhasree Roy Choudhury*, (2020) Nanomedicine: Nanotechnology, Biology, and Medicine, 24: 102088. DOI: 10.1016/j.nano.2019.102088.

  4. κ-carrageenan-C phycocyanin based smart injectable hydrogels for accelerated wound recovery and real-time monitoring.: Atul Dev,Soni J Mohanbhai,Avinash C. Kushwaha,Mohammed Nadim Sardoiwala ,Subhasree Roy Choudhury* ,Surajit Karmakar*, (2020) Acta Biomaterialia, 109: 121-131. DOI: https://doi.org/10.1016/j.actbio.2020.03.023

  5. Hypericin-Loaded Transferrin Nanoparticles Induce PP2A-Regulated BMI1 Degradation in Colorectal Cancer-Specific Chemo-Photodynamic Therapy: Mohammed Nadim Sardoiwala, Avinash Chandra Kushwaha, Atul Dev, Nishith Shrimali, Prasenjit Guchhait, Surajit Karmakar* , and Subhasree Roy Choudhury*, (2020) ACS Biomater. Sci. Eng., 6: 3139-3153. DOI: https://doi.org/10.1021/acsbiomaterials.9b01844

  6. Near-Infrared Responsive Dopamine/Melatonin-Derived Nanocomposites Abrogating in Situ Amyloid β Nucleation, Propagation, and Ameliorate Neuronal Functions.: Srivastava AK, Roy Choudhury S*, Karmakar S*, (2020) ACS Appl. Mater. Interfaces, 12: 5658-5670. DOI: https://doi.org/10.1021/acsami.9b22214

  7. Paclitaxel nanocrystalline assemblies as a potent Transcatheter arterial chemoembolization (TACE) candidate for unresectable hepatocellular carcinoma.: Dev A, Sood A, Roy Choudhury S, Karmakar S*, (2020) Materials Science & Engineering C, 107: 110315.. DOI: https://doi.org/10.1016/j.msec.2019.110315

  8. Nanoformulation of EPZ011989 attenuates EZH2-c-Myb epigenetic interaction by proteasomal degradation in Acute Myeloid Leukemia.: Kaundal B; Srivastava A; Dev A; Mohanbhai Soni J; Karmakar S; Roy Choudhury S*, (2020) Mol. Pharmaceutics, 2: 604-621. DOI: https://doi.org/10.1021/acs.molpharmaceut.9b01071

  9. Disulfide-Bridged Chitosan-Eudragit S-100 Nanoparticles for Colorectal Cancer.: Sood A, Dev A, Mohanbhai SJ, Shrimali N, Kapasiya M, Kushwaha A, Roy Choudhury S, Guchhait P, Karmakar S*., (2019) ACS Appl. Nano Mater, 2, 10: 6409-6417.. DOI: https://doi.org/10.1021/acsanm.9b01377

  10. Nanostructure Endows Neurotherapeutic Potential in Optogenetics: Current Development and Future Prospects.: Mohammed Nadim Sardoiwala, Anup K. Srivastava, Surajit Karmakar*, Subhasree Roy Choudhury*., (2019) ACS Chem. Neurosci, 10, 8: 3375-3385.. DOI: https://doi.org/10.1021/acschemneuro.9b00246

  11. NIR-responsive Indocyanine green-GenisteinNanoformulation controlling Polycomb epigenetic machinery for efficient Photo-Chemo-Combotherapy of Glioblastoma.: Kaundal B, Srivastava AK, Sardoiwala N Md, Karmakar S, Roy Choudhury S*, (2019) Nanoscale Advances, 1: 2188-2207. DOI: https://doi.org/10.1039/C9NA00212J

  12. Development of engineered nanoparticles expediting diagnostic and therapeutic applications across blood brain barrier.: Mohammed Nadim Sardoiwala Babita Kaundal and Subhasree Roy Choudhury*, (2018) Handbook of Nanomaterials for Industrial Applications, Elsevier Press, 2018: 696-709. DOI: https://doi.org/10.1016/B978-0-12-813351-4.00038-9

  13. New Insight into Curcumin Tethered Lanthanum Carbonate Nanospheres and Protein Corona Conferring Fluorescence Enhancement based Sensitive Detection of Amyloid-beta: Srivastava AK, Dev A, Karmakar S, (2018) Sensors and Actuators B: Chemical, 262: 687-695. DOI: 10.1016/j.snb.2018.02.002

  14. Superior Bactericidal Efficacy of Fucose-Functionalized Silver Nanoparticles against Pseudomonas aeruginosa PAO1 and Prevention of Its Colonization on Urinary Catheters.: Bhargava A, Pareek V,Panwar J, Karmakar S., (2018) ACS Appl Mater Interfaces, 10(35): 29325-29337. DOI: 10.1021/acsami.8b09475

  15. 1,3β Glucan anchored, paclitaxel loaded chitosan nanocarrier endows enhanced hemocompatibility with efficient anti-glioblastoma stem cells therapy: Pankaj K. Singh,Anup K. Srivastava,Atul Dev,Babita Kaundal,Subhasree Roy Choudhury* ,Surajit Karmakar*, (2018) CarbohydrPolym, 180: 365-375. DOI: 10.1016/j.carbpol.2017.10.030

  16. Nanomaterial toxicity in Microbes, Plants and Animals.: Kaundal B, Dalai S, (2017) Nanoscience in Food and Agriculture, (Springer Nature publisher)., 5: 243-266. DOI: 10.1016/j.bbrc.2010.04.156

  17. Nanomelatonin triggers superior anticancer functionality in a human malignant glioblastoma cell line.: Kumar Yadav S, Kumar Srivastava A, Dev A, Kaundal B, Karmakar S*., (2017) Nanotechnology, 28: 365102. DOI: 10.1088/1361-6528/aa7c76

  18. Toxic impact of nanomaterials on microbes, plants and animals.: Sardoiwala MN, Kaundal B, (2017) Environ Chem Lett., 16: 147-160. DOI: 10.1007/s10311-017-0672-9

  19. Nano-curcumin influences blue light photodynamic therapy for restraining glioblastoma stem cells growth: Dev A, Srivastava AK, Karmakar S, (2016) RSC Adv., 6: 95165-95168. DOI: 10.1039/C6RA20269A

  20. Valproic Acid Induced Differentiation and Potentiated Efficacy of Taxol and Nanotaxol for Controlling Growth of Human Glioblastoma LN18 and T98G Cells: Karmakar S, Banik NL, Ray SK, (2011) Neurochem Res, 36: 2292-305. PMID: 21786169.. DOI: 10.1007/s11064-011-0554-7

  21. Epigallocathechin-3-gallate and DZNep reduce polycomb protein level via a proteasome-dependent mechanism in skin cancer cells: Balasubramanian S, Chew Yap, Han B, Marquez V, Eckert RL, (2011) Carcinogenesis, 32: 1525-32. PMID: 21798853.. DOI: 10.1093/carcin/bgr171

  22. N-(4-Hydroxyphenyl) Retinamide Potentiated Anti-Tumor Efficacy of Genistein in Human Ewing’s Sarcoma Xenografts.: Karmakar S, Choudhury SR, Banik NL, Ray SK, (2011) World J Oncol, 2: 53-63. PMID:21822457. DOI: 10.4021/wjon301w

  23. Induction of Mitochondrial Pathways and Endoplasmic Reticulum Stress for Increasing Apoptosis in Ectopic and Orthotopic Neuroblastoma Xenografts.: Karmakar S, Banik NL, Ray SK, (2011) J Cancer Ther, 2: 77-90,PMID:22468231. DOI: 10.4236/jct.2011.22009

  24. Activation of Multiple Molecular Mechanisms for Increasing Apoptosis in Human Glioblastoma T98G Xenograft.: Karmakar S, Banik NL, Ray SK, (2010) J Cancer Sci Ther, 2: 107-113, PMID: 21666767. DOI: 10.4172/1948-5956.1000033

  25. Down regulation of angiogenic and survival factors increased apoptosis through upregulation of p53 and p21 in malignant neuroblastoma cells having N-Myc amplification or non-amplification.: Karmakar S, Banik NL, Ray SK, (2010) Investigational New Drugs, 28: 812-24. PMID: 19777160.. DOI: 10.1007/s10637-009-9324-7

  26. Combination of N-(4-hydroxyphenyl) retinamide and genistein increased apoptosis in neuroblastoma SK-N-BE2 and SH-SY5Y xenografts: Karmakar S, Banik NL, Ray SK, (2009) Neuroscience, 163: 286-295. PMID: 19540315.. DOI: 10.1016/j.neuroscience.2009.06.037

  27. Bcl-2 inhibitor and apigenin worked synergistically in human malignant neuroblastoma cell lines and increased apoptosis with activation of extrinsic and intrinsic pathways.: Karmakar S, Davis KA, Deeconda A, Banik NL, Ray SK, (2009) BBRC, 388: 705-710,PMID: 19695221.. DOI: 10.1016/j.bbrc.2009.08.071

  28. Bcl-2 inhibitor and apigenin worked synergistically in human malignant neuroblastoma cell lines and increased apoptosis with activation of extrinsic and intrinsic pathways.: Karmakar S, Davis KA, Deeconda A, Banik NL, Ray SK, (2009) BBRC, 388: 705-710,PMID: 19695221.. DOI: 10.1016/j.bbrc.2009.08.071

  29. Bcl-2 inhibitor HA14-1 and genistein together adeptly down regulated survival factors and activated cysteine proteases for apoptosis in human malignant neuroblastoma SK-N-BE2 and SH-SY5Y cell: Nishant Mohan, Naren L. Banik, and Swapan K. Ray, (2009) Brain Res, 1283,: 155-166. PMID: 19505441.. DOI: 10.1016/j.brainres.2009.05.097

  30. A heat stable protein toxin (drCT-I) from the Indian Viper (Daboia russelli russelli) venom having antiproliferative, cytotoxic and apoptotic activities.: A Gomes, A Saha, R Mishra, B Giri, AK Biswas, A Debnath, A Gomes, (2007) Toxicon, 49(1): 46-56, PMID: 17055549.. DOI: 10.1016/j.toxicon.2006.09.009

  31. Purification, crystallization and preliminary X-ray structural studies of a 7.2 kDa cytotoxin isolated from the venom of Daboia russelli russelli of the Viperidae family.: Aparna Gomes, Antony Gomes, Jiban K. Dattagupta,Udayaditya Sen, (2006) Acta Cryst., F62: 292–294. PMID: 16511326.. DOI: 10.1107/S1744309106005963

  32. Hepatoprotective and Immunomodulatory properties Of Tinospora cordifolia in CCL4 intoxicated mature albino rats.: B.Bishayi,S.Ghose,M.Sengupta, (2002) The Journal Of Toxicological Science, 27(3): 139-146. PMID: 12238138.. DOI: 10.2131/jts.27.139

  1. Recuperative Effect of Metformin loaded Polydopamine Nanoformulation promoting EZH2 mediated Proteasomal Degradation of phospho-α-Synuclein in Parkinson's disease model: Md Nadim Sardoiwala ,Anup K Srivastava ,Babita Kaundal ,Surajit Karmakar ,Subhasree Roy Choudhury*, (2020) Nanomedicine: Nanotechnology, Biology, and Medicine, 24: 102088. DOI: 10.1016/j.nano.2019.102088.

  1. Nanobiosensors and Its Application in Agriculture and Food. Nanopharmaceuticals,: Sardoiwala N Md, Srivastava AK, Roy Choudhury S, Karmakar S*., (2020) 21st Century Nanoscience – A Handbook; Nanopharmaceuticals, Nanomedicine, and Food Nanoscience;Taylor and Francis Group, 1st Ed, Volume 8: 344. DOI: https://doi.org/10.1201/9780429351587

  2. Role of angiogenesis in the pathogenesis of glioblastoma and antiangiogenic therapies for controlling glioblastoma. In Glioblastoma: Molecular Mechanisms of Pathogenesis and Current Therapeut: Karmakar S, Banik NL, Ray SK, (2010) Publisher: Springer; 1 edition,, 217-241 , ISBN-978-1-4419-0409-6..

  1. c-Myb interaction with histone acetyltransferase p300 regulates its transcriptional activities in human leukaemic cells.: New directions in leukemia research. March 30-April 2nd, Noosa, QLD, Australia. Ding Jianmin, Cao Lu, and Gonda Tom, (2014) .

  2. Functional validation of Myb target genes in myeloid leukaemic transformation. New directions in leukemia research.: March 30-April 2nd, Noosa, QLD, Australia. Gonda Tom, (2014) .

  3. Synergistic efficacy of HA14-1 and apigenin in inhibiting angiogenic factors and increasing apoptosis in neuroblastoma cells.: Frontiers in Neuroscience Conference. March 05 - 07, Atlanta, USA Davis KA, Karmakar S, Banik NL and Ray SK, (2010) .

  4. Combination of valproic acid and taxol or nanotaxol effectively inhibited angiogenic factors and increased apoptosis in glioblastoma U87MG cells in culture and xenografts in nude mice.: Presented at the 40th Annual ASN Meeting, Charleston, SC - March 7-11 Karmakar S, Banik NL, Ray SK, (2009) .

  5. Combination of sorafenib and genistein inhibits angiogenesis and triggers multiple molecular mechanisms for apoptosis in human malignant neuroblastoma SK-N-DZ and SH-SY5Y cell lines presented: Karmakar S, Banik NL, Ray SK, (2009) .

  6. Activation of both extrinsic and intrinsic pathways of apoptosis in human neuroblastoma SH-SY5Y cells in culture as well as in xenografts in athymic nude mice.: Presented at the 40th Annual ASN Meeting, Charleston, SC - March 7-11 Karmakar S, Banik NL, Ray SK, (2009) .

  7. Combination of N-(4-hydroxyphenyl) retinamide and genistein increases apoptosis in Ewing’s sarcoma SK-N-MC cells in culture and xenografts in athymic nude mice.: Presented at the 100th AACR Annual Meeting Denver, CO, April 18-22, Karmakar S, Tomlinson S, Banik NL, Ray SK, (2009) .

  8. Combination of Bcl-2 inhibitor and apigenin activated extrinsic and intrinsic pathways of apoptosis in neuroblastoma SK-N-DZ cells.: Presented at the 40th Annual ASN Meeting, Charleston, SC - March 7-11, Davis K, Karmakar S, Deeconda A, Banik NL, Ray SK, (2009) .

  9. Small Molecule Inhibitor of Bcl-2 Enhances Efficacy of Genistein for Apoptosis in Neuroblastoma SH-SY5Y and SK-N-BE2 Cell lines.: Mohan N, Karmakar S, Banik NL , Ray SK, (2009) .

  10. Combination of valproic acid and taxol or nanotaxol induced differentiation and apoptosis in glioblastoma LN18 and T98G cells presented at the 40th Annual ASN Meeting,: Charleston, SC - March 7-11 Karmakar S, Banik NL, Ray SK, (2009) .

  11. A new protein toxin (drCT1) from Dabuia russellii venom having antiproliferative and apoptogenic activity in15th World Congress on Animal,: Plant & Microbial Toxins, International Society on Toxinology, Glasgow, UK. July 27. Dasgupta SC, Saha A, Giri B, Gomes A, (2006) .

  12. Structure and functional characterization of a heat stable antineoplastic protein toxin (Toxin CM28) from Russells viper venom.: 37th Annual Conference of the Indian Pharmacological Society, Kolkata, January 14-16. Gomes A, (2005) .

  13. Antineoplastic protein toxin (Toxin CM28) from Vipera russelli russelli venom.: 38th Annual Conference of the Indian Pharmacological Society, Chennai, December 28-30. Gomes A, (2005) .

  14. Pharmacological characterization of a heat stable protein toxin (Toxin CM 28) from Russells viper venom CM 28) from Russells viper venom.: 36th Annual Conference of the Indian Pharmacological Society, Delhi, December, 5-7. Gomes A, (2003) .

  15. Hepatoprotective, immunostimulatory and antioxidant activities of Tinospora cordifolia in CCL4 intoxicated mature albino rats. XIII Annual Conference of the Physiological Society of India,: Kolkata, February 23-24. Bishayi B, (2002) .

Fundings

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  • 2007

    Ph.D

    University of Calcutta, India (Subject: Physiology; awarded 2009)

  • 2002

    SLET,GATE

    Qualified

  • 2001

    M.Sc

    University of Calcutta, India (Subject: Physiology).

  • 1999

    B.Sc

    University of Calcutta, India (Subject: Physiology) (Honors), Chemistry, Zoology.

  • Post Doctoral Fellow:The School of Pharmacy, Pharmacy Australia Centre of Excellence, The University of Queensland, QLD 4102,, Australia (April 2012 to March 2014 )

  • Post Doctoral Fellow:Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201,, USA (May 2010 to April 2011 )

  • Post Doctoral Fellow:Department of Pathology, Microbiology and Immunology, University of South Carolina, Columbia, SC 29209,, USA (March 2008 to March 2010 )

  • Post Doctoral Fellow:Department of Neurosciences, Medical University of South Carolina, Charleston, SC 29425,, USA. (March 2007 to November 2007 )

Awards & Honours

  • Awarded Gold Medal by University of Calcutta for securing the 1st Class 1st rank in M.Sc Physiology in 2001.

  • Awarded with Dr. D.N. Mallick Memorial Gold Medal by the Physiological Society of India for securing the 1st Class 1st rank in M.Sc Physiology in 2001.

  • Awarded with Prof. A. K. Mukherjee Memorial Cup by the 6th Annual Scientific Meet Committee, Calcutta in B.Sc examination in Physiology in 1999.

  • Awarded National Scholarship by the West Bengal Board of Secondary Education in Madhyamik examination in 1994.

  • Awarded Junior Research Fellowship and Senior Research Fellowship by UGC.


Professional Recognitions