Faculty Directory
Dr. Manish Singh
Assistant Professor (Scientist-D), Chairman, IOLC
Our research group is dedicated to unraveling the complex interactions between environmental pollutants, nanomaterials, and biological systems. Our multidisciplinary approach spans from evaluating the toxicological and environmental impacts of nanoplastics and nanopollutants—such as those derived from single-use PET bottles and textile dyes—to investigating the molecular mechanisms underlying chemical-induced apoptosis and antibiotic resistance in bacteria. Additionally, we explore innovative strategies for neural regeneration and neuroprotection, addressing challenges in neuroinflammation, neurodegeneration in conditions like Parkinson's disease, and developmental neurotoxicity following prenatal exposure to pharmaceuticals. By integrating advanced nanotechnology, in vitro toxicity assessments, and natural therapeutics, our work aims to develop novel interventions for environmental remediation and improved human health outcomes.
Contact Information :
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Email:
manish@inst.ac.in -
Google Link:
Google Link
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Our group is engaged in a broad spectrum of research areas, including cellular and molecular neuroscience, in utero exposures and central nervous system (CNS) development, and neuro-behavioral analysis. We also focus on emerging fields such as nanoneurotoxicology, developmental nanotoxicology, and nano environmental health and safety. Additionally, we develop and utilize advanced bioimaging techniques, including confocal and electron microscopy, to further our understanding of these complex biological processes.
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Our research on nanomaterials-induced neuromodulation focuses on understanding how engineered nanomaterials can modulate neural activity, offering promising therapeutic strategies for neurodegenerative disorders and neural injuries. We are advancing with a two-pronged approach. First, we are exploring emerging strategies for neural regeneration by harnessing physical stimulation—either as a standalone method or in combination with smart piezoelectric nano-biomaterials—to promote neural repair and functional recovery. Second, we are advancing our understanding of neuronal modulation using semiconducting graphitic carbon nitride, integrating insights from in vitro, in vivo, and in silico studies to elucidate its mechanisms and therapeutic potential.
Our contribution in the field:
1. De et. al., ACS Appl. Mater. Interfaces 2025, 17 (7), 10387-10401,
2. De et. al., Eur. J. Pharm. Biopharm. 173 (2022) 73–91, -
Our research interest centers on unraveling the complex interactions between nanomaterials and their impacts on both environmental and public health. Currently, we are focusing on two critical areas:
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Antibiotic Resistance Propagation by Nanoplastics:
We are investigating how nanoplastics derived from single-use polyethylene terephthalate (PET) bottles contribute to the spread of antibiotic resistance in bacteria. Our research delves into the mechanisms by which these nanoplastics induce genetic transformation and stimulate the secretion of outer membrane vesicles, thereby facilitating the horizontal transfer of resistance genes. -
Toxicological and Environmental Impact of Nanopollutants:
In parallel, we are evaluating the broader toxicological and environmental implications of nanopollutants. This research aims to understand how these emerging contaminants interact with ecosystems and affect human health, providing essential insights for risk assessment and mitigation strategies.
Through these studies, we aim to provide a comprehensive understanding of how nanomaterials influence microbial dynamics and environmental safety, ultimately contributing to the development of innovative solutions to counteract their adverse effects.
Our Contribution in the field:1. Sharma et. al., Nanoscale 2024, 16 (46), 21360-21378,
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Our current research focuses on the toxicological assessment and biodistribution of nanomaterials, specifically pristine graphene oxide (GO) nanoflakes. We are conducting short-term in vivo studies in SD rats to evaluate how these nanoflakes distribute throughout the body following intravenous injection and to determine their acute toxicity profiles. A major emphasis of our work is on the hazardous effects of GO on reproductive health, fetal development, and long-term behavioral outcomes. By investigating these aspects, we aim to elucidate the potential risks associated with GO exposure, thereby providing a comprehensive understanding of its impact on critical biological systems. Our findings are expected to inform safer nanomaterial design and guide regulatory strategies for their biomedical and industrial applications.
Our Contribution in the field:
De et. al., Tox. Res. 2024, 13 (2),
Current Group Members
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MR. INDRANIL DE
Email: indranil.ph19227@inst.ac.in
Reg. No.: PH19227
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MR. PRASHANT SHARMA
Email: prashant.ph19237@inst.ac.in
Reg. No.: PH19237
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MR. GULSHAN KUMAR
Email: gulshan.ph21225@inst.ac.in
Reg. No.: PH21225
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DR KANIKA PHUTELA
Email:
Reg. No.:
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DR. ABHINOY KISHORE
Email: abhinoy.ra202120@inst.ac.in
Reg. No.: RA202120
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KARAMJEET KAUR
Email:
Reg. No.:
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MANISH BHARDWAJ
Email:
Reg. No.:
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HENNA M. WANI
Email:
Reg. No.:
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SAKSHI DAGARIYA
Email:
Reg. No.: IN-2024/28
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MR RAJESH
Email:
Reg. No.:
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MANISH R
Email:
Reg. No.:
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SAIQA
Email:
Reg. No.:
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NISHI KUMARI
Email:
Reg. No.:
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AAKRATI MEHRA
Email:
Reg. No.:
Present Affiliation: Pursuing PhD from IITK
Alumni
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1.
Single-use polyethylene terephthalate bottle-derived nanoplastics propagate antibiotic resistance in bacteria via transformation and outer membrane vesicle secretion , Prashant Sharma, Abhinoy Kishore and Manish Singh* , Nanoscale , 2025 , 16 (46) , 21360-21378 (IF: 5.8) , https://doi.org/10.1039/D4NR02613F -
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Advanced Neuronal Modulation with Semiconducting Graphitic Carbon Nitride: Insights from In Vitro, In Vivo, and In Silico Studies: , Indranil De, Abhinoy Kishore, Subhabrata Das, Sownyak Mondal, Sakshi Yadav, Prashant Sharma, Mansi Pahuja, Srishti Singh, Aamir Nazir*, Soumya Ghosh*, Kaushik Ghosh* and Manish Singh* , ACS Applied Materials & Interfaces , 2025 , 17(7) , 10387–10401 (IF: 8.5) , https://doi.org/10.1021/acsami.4c19242 -
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Isolation of Amyloid-like Protein Aggregates (APA) from white bread and their characterisation , Abhishek Singh Yadav, Shweta Malik, Indranil De, Bhumika Pippal, Manish Singh, Neha Jain, Jay Kant Yadav* , Biophysical Chemistry , 2024 , 302 , 107097 (IF: 3.8) , https://doi.org/10.1016/j.bpc.2023.107097 -
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Synergistic potential of α-Phellandrene combined with conventional antifungal agents and its mechanism against antibiotic resistant Candida albicans , Riya Bhattacharya, Prashant Sharma, Debajyoti Bose*, Manish Singh* , CABI Agriculture and Bioscience , 2024 , 5 (1) , https://doi.org/10.1186/s43170-024-00218-1 -
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Short term biodistribution and in vivo toxicity assessment of intravenously injected pristine graphene oxide nanoflakes in SD rats , Indranil De , Rashmika Singh , Sushil Kumar , Srishti Singh , Manohar Singh , Jiban Jyoti Panda , Kaushik Ghosh , Durga Prasad Mishra , Manish Singh* , Toxicology Research , 2024 , 13 (2) , (IF: 2.28) , 10.1093/toxres/tfae058 -
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Lipopolysaccharide targeting-peptide-capped chitosan gold nanoparticles for laser-induced antibacterial activity , Samraggi Choudhury, Aakrati Mehra, Swapnil Srivastava, Manju Sharma, Manish Singh* and Jiban Jyoti Panda* , Nanomedicine , 2024 , 19(23) , 1913-1929 (IF: 4.7) , 10.1080/17435889.2024.2382073 -
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Mitigating neuroinflammation in Parkinson's disease: Exploring the role of proinflammatory cytokines and the potential of phytochemicals as natural therapeutics , Prashant Sharma, Abhinoy Kishore, Indranil De, Swarnima Negi, Gulshan Kumar, Sahil Bhardwaj and Manish Singh* , Neurochemistry International , 2023 , 105604 , (IF: 4.2) , 10.1016/j.neuint.2023.105604 -
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Emerging approaches of neural regeneration using physical stimulations solely or coupled with smart piezoelectric nano-biomaterials , Indranil De, Prashant Sharma and Manish Singh* , European Journal of Pharmaceutics and Biopharmaceutics 2022 , 2023 , Volume 173 , 73-91 (IF: 4.4) , https://doi.org/10.1016/j.ejpb.2022.02.016 -
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In-vitro toxicity assessment of a textile dye Eriochrome Black T and its nano-photocatalytic degradation through an innovative approach using Mf-NGr-CNTs-SnO2 heterostructures , Indranil De, Mansi Pahuja, Henna Mohi Ud Din Wani, Akashdeep Dey, Taru Dube, Rishita Ghosh, Nimisha Kankan, Jibanananda Mishra, Jiban Jyoti Panda, Takahiro Maruyama, Kaushik Ghosh, Manish Singh* , Ecotoxicology and Environmental Safety , 2022 , Volume 243 , 113985 (IF: 6.2) , 10.1016/j.ecoenv.2022.113985 -
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Exposure of calcium carbide induces apoptosis in mammalian fibroblast L929 cells , Indranil De, Rajesh S, Avneet Kour, Henna Wani, Prashant Sharma, Jiban Jyoti Panda, Manish Singh* , Toxicol Mech Methods , 2021 , 31(3) , 159-168 (IF 2.29) , https://doi.org/10.1080/15376516.2020.1849484 -
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Isolation and Characterisation of Milk-derived Amyloid-like Protein Aggregates (MAPA) from Cottage Cheese: , Shweta Malik, Indranil De, Manish Singh, Charis M Galanakis, Abdulhakeem S Alamri, Jay Kant Yadav , 2021 , Food Chemistry , 373: 131486 -
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Effects of prenatal exposure to antipsychotic risperidone on developmental neurotoxicity, apoptotic neurodegeneration and neurobehavioral sequelae in rat offspring : , Singh KP, Singh MK, Singh Manish , 2016 , 52: 13-23 , Int J Dev Neurosci , 10.1016/j.ijdevneu.2016.05.006 -
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Efficient MoS2 exfoliation by cross beta amyloid nanotubes for multi stimuli responsive and biodegradable aqueous dispersions : , Nidhi Kapil, Asmeet Singh, Manish Singh, Dibyendu Das. , 2016 , 55(27): 7772-6. , Angew Chem Int Ed Engl , 10.1002/anie.201509953 -
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Assessment of in-utero Venlafaxine induced, ROS-mediated, apoptotic neurodegeneration in fetal neocortex : , Manish Singh, K.P. Singh, Shubha Shukla , Madhu Dikshit. , 2015 , 40: 60-91. (IF-2.58) , Int J Dev Neurosci. , 10.1016/j.ijdevneu.2014.10.007 -
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Effect of galvanotaxic graphene oxide on chloroplast activity: Interaction quantified with Biolayer-Interferometry coupled confocal microscopy: , Sharma S., Sahu B.K., Srinivasan S., Singh M., G. Jayamurugan., Vijayakumar S.* , (2020) , 162: 147-156 , Carbon , 10.1016/j.carbon.2020.02.054 -
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Gold Nano-/Microroses on Levodopa Microtubes for SERS-Based Sensing of Gliomas: , T Dube, N Kumar, A Kour, J Mishra, Manish Singh, B Prakash, JJ Panda , (2019) , 2 (5): 2663-2678 , ACS Applied Nano Materials , 10.1021/acsanm.9b00155 -
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In Utero Exposure of Venlafaxine: Impact on maternal, fetal, postnatal growth and possible birth defects in rat offspring: , Manish Singh, K.P. Singh , (2013) , 36 (1): 35-40 (IF-0.29) , Natl Acad Sci Let. , 10.14336/AD.2014.05001 -
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Consequences of Alteration in Leucine Zipper Sequence of Melittin in Its Neutralization of Lipopolysaccharide-induced Proinflammatory Response in Macrophage Cells and Interaction with Lipopol: , aghvendra M. Srivastava, Saurabh Srivastava, Manish Singh, Virendra Kumar Bajpai,Jimut Kanti Ghosh , (2012) , 287(3): 1980-95 (IF-4.57) , J. Biol. Chem. , 10.1074/jbc.M111.302893 -
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Inducing toxicity by introducing a leucine-zipper-like motif in frog antimicrobial peptide, magainin 2: , Brijesh Kumar Pandey, Saurabh Srivastava,Jimut Kanti Ghosh. , (2011) , 436: 609–620(IF-4.39). , Biochem. J. , 10.1042/BJ20110056 -
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Effect of hypothyroxinemia on thyroid hormone responsiveness and action during rat postnatal neocortical development : , Satish Babu, Rohit Anthony Sinha, Vishwa Mohan, Geeta Rao, Amit Pal, Amrita Pathak, Madan M. Godbole , (2011) , 228(1): 91-98. IF-4.69 , Exp. Neurol. , 10.1016/j.expneurol.2010.12.012 -
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Functional and Molecular Characterization of NOS Isoforms in Rat Neutrophil Precursor Cells : , Anupam Jyoti, Ravi Shankar Keshari,Manoj Kumar Barthwal, Madhu Dikshit.,Sachin Kumar , (2010) , 77(5): 467-77 (IF-2.9) , Cytometry A , 10.1002/cyto.a.20852 -
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Isolation and characterization of amyloid-like protein aggregates from soya beans and the effect of low pH and heat treatment on their stability , Neelam Jangir, Shreya Bangrawa, Tamanna Yadav, Shweta Malik, Abdulhakeem S. Alamri, Charis M. Galanakis, Manish Singh*, Jay Kant Yadav* , Journal of Food Biochemistry 2022 , 46 (10) , e14369 (IF: 3.65) , https://doi.org/10.1111/jfbc.14369 -
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Prenatal Venlafaxine Exposure-Induced Neurocytoarchitectural and Neuroapoptotic Degeneration in Striatum and Hippocampus of Developing Fetal Brain, Manifesting Long-term Neurocognitive Impairments in Rat Offspring , K P Singh, Prashant Sharma and Manish Singh* , Neurotoxicity Research 2022 , 40 (5) , 1174-1190 (IF: 3.98) , https://doi.org/10.1007/s12640-022-00541-3 -
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Cost-effective microreactors for the synthesis of SnS nanoparticles and inline photocatalytic degradation of azo dyes , Vibhav Katoch, Manish Singh, Akash Katoch, Bhanu Prakash* , Materials Letters 2023 , Volume 333 , 133677 (IF:3.57) , https://doi.org/10.1016/j.matlet.2022.133677 -
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Bioenhancer potential of Aegle marmelos (L.) Corrêa essential oil with antifungal drugs and its mode of action against Candida albicans , Riya Bhattacharya, Anuradha Sourirajan, Prashant Sharma, Anil Kumar, Navneet Kumar Upadhyay, Rakesh Kumar Shukla, Kamal Dev, Balaji Krishnakumar, Manish Singh*, Debajyoti Bose* , Biocatalysis and Agricultural Biotechnology 2023 , Volume 48 , 102647 (IF: 3.4) , https://doi.org/10.1016/j.bcab.2023.102647 -
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Miniatured Fluidics-Mediated Modular Self-Assembly of Anticancer Drug-Amino Acid Composite Microbowls for Combined Chemo-Photodynamic Therapy in Glioma , Sonika Chibh, Vibhav Katoch, Manish Singh, Bhanu Prakash*, Jiban Jyoti Panda* , ACS Biomaterials Science & Engineering 2021 , 7(12) , 5654-5665 (IF 5.5) , https://doi.org/10.1021/acsbiomaterials.1c01023 -
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Heat treatment of soluble proteins isolated from human cataract lens leads to the formation of non-fibrillar amyloid-like protein aggregates , Chandrika Mittal, Ashwani Kumari, Indranil De, Manish Singh, Ramswaroop Harsolia, Jay Kant Yadav* , International Journal of Biological Macromolecules 2021 , 188 (1) , 512-522 , https://doi.org/10.1016/j.ijbiomac.2021.07.158 -
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Exploration of silicate solubilizing bacteria for sustainable agriculture and silicon biogeochemical cycle , Gaurav Raturi, Yogesh Sharma, Varnika Rana, Vandana Thakral, Balaraju Myaka, Prafull Salvi, Manish Singh, Hena Dhar*, Rupesh Deshmukh* , Plant Physiology and Biochemistry 2021 , 166 , 827-838 (IF: 6.1) , 10.1016/j.plaphy.2021.06.039 -
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Disaggregation of amyloid-like protein aggregates isolated from human cataract lens , Chandrika Mittal, Ram Swaroop Harsolia, Manish Singh, Jay Kant Yadav* , Indian Journal of Biochemistry and Biophysics (IJBB) , 58(4) , 359-365 , https://doi.org/10.56042/ijbb.v58i4.51476 -
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Seamless architecture of porous carbon matrix decorated with Ta2O5 nanostructure-based recyclable photocatalytic cartridge for toxicity remediation of industrial dye effluents , Mansi Pahuja, Indranil De, Shumile Ahmed Siddiqui, Subhabrata Das, Mohd Afshan, Kehkashan Alam, Sk Riyajuddin, Seema Rani, Rishita Ghosh, Daya Rani, Komal Gill, Manish Singh*, Kaushik Ghosh* , Separation and Purification Technology 2023 , 123685 , (IF: 9.1) , https://doi.org/10.1016/j.seppur.2023.123685 -
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Modulation of protein phosphatase 1 gamma 2 during cell division of cervical cancer HeLa cells , Saurabh Kumar Agnihotri*, Parmita Kar*, Manish Singh, Garima Pant, Kalyan Mitra, Madan Lal Brahma Bhatt, Monika Sachdev , Wspólczesna Onkologia 2021 , 25 (2) , 125 (IF 1.81) , https://doi.org/10.5114/wo.2021.107745 -
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Microflow synthesis and enhanced photocatalytic dye degradation performance of antibacterial Bi2O3 nanoparticles , Vibhav Katoch, Nipun Sharma, Manju Sharma, Mayank Baghoria, Jiban Jyoti Panda, Manish Singh, Bhanu Prakash* , Environmental Science and Pollution Research 2021 , 28 , 19155–19165 (IF 4.22) , https://doi.org/10.1007/s11356-020-11711-1 -
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Continuous flow fabrication of Fmoc-cysteine based nanobowl infused core–shell like microstructures for pH switchable on-demand anti-cancer drug delivery , Sonika Chibh, Vibhav Katoch, Avneet Kour, Farheen Khanam, Amit Singh Yadav, Manish Singh, Gopal C. Kundu, Bhanu Prakash* and Jiban Jyoti Panda* , Biomaterials Science 2021 , 9(3) , 942-959 , https://doi.org/10.1039/D0BM01386B -
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A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM , Sandeep Sharma, Mohd. Muddassir, Saraladevi Muthusamy, Pardeep Kumar Vaishnav, Manish Singh, Deepak Sharma, Selvaraju Kanagarajan* & Vijayakumar Shanmugam* , Scientific reports 2020 , 10(1) , 19233 , https://doi.org/10.1038/s41598-020-75685-3 -
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Predicted aggregation-prone region (APR) in βB1-crystallin forms the amyloid-like structure and induces aggregation of soluble proteins isolated from human cataractous eye lens , Ram Swaroop Harsolia, Ambika Kanwar, Shalini Gour, Vijay Kumar, Vikas Kumar, Rati Bansal, Suman Kumar, Manish Singh, Jay Kant Yadav* , International Journal of Biological Macromolecules 2020 , 163 , 702-710 , https://doi.org/10.1016/j.ijbiomac.2020.07.028
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Breaking the Barriers of Nanotoxicological Assessments: The Importance of Available Models and Future Perspectives , Abhinoy Kishore, Indranil De, Prashant Sharma and Manish Singh* , 22 , 163-184 , 10.2174/9789815123555123010011
I have amassed nearly two decades of research experience across diverse academic and scientific institutions. My career began as a Research Scholar at the University of Allahabad, Prayagraj (2006–2008), where I honed my foundational research skills. I then joined CSIR-CDRI, Lucknow as a Technical Officer (2008–2014), contributing to various research projects. In 2014, I transitioned to INST Mohali, where I advanced from Scientist-B (2014–2018) to Scientist-C (2018–2022). Currently, I serve as Scientist-D at INST Mohali (2022–present), continuing to drive innovative research and contribute to scientific advancements.
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Title: Glioma Targeting Anticancer Peptide Theranostic Nanoparticles
CO-PI: Dr. Manish Singh
Tenure: 3 years
Funding Agency: DBT Biocare
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Title: Assessment of maternal, reproductive and fetal toxicity induced by intrauterine exposure of carbonaceous nanomaterials like graphene and carbon nanotubes
PI: Dr. Manish Singh
Funding Agency: SERB (DST)
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Title: Pragmatic strategies for the delivery of phytodrugs across BBB through plant derived exosomes for the management of neuroinflammatory diseases
PI: Dr Manish Singh
Funding Agency: DST AISTIC
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Title: Noninvasive and in situ forming peptide-polymer hydrogel containing CPP decorated nanoparticles
CO-PI: Dr Manish Singh
Funding Agency: ICMR