Photodynamic therapy with CNQDs

Nontoxic Metal-Free Visible Light-Responsive Carbon Nitride Quantum Dots Cause Oxidative Stress and Cancer-Specific Membrane Damage

Graphitic carbon nitride (also known as g-CN or g-C3N4) has the intrinsic ability to generate electron–hole pairs under visible light illumination, resulting in the generation of reactive oxygen species (ROS). We report g-CN quantum dots (g-CNQDs) as a standalone photodynamic transducer for imparting significant oxidative stress in glioma cells, manifested by the loss of mitochondrial membrane potential. With an optimized treatment time, visible light source, and exposure window, the photodynamic treatment with g-CNQDs could achieve 90% cancer cell death via apoptosis. The g-CNQDs, otherwise biocompatible with normal cells up to 5 mg/mL, showed 20% necrotic cancer cell death in the absence of light due to membrane damage induced by a charge shielding effect at the acidic pH prevailing in the tumor environment. Acute toxicity analysis in C57BL/6 mice with intravenously injected g-CNQDs at a 20 mg/kg dose showed no signs of inflammatory response or organ damage.

More info: Pranjali Yadav, Mimansa, Kamalakannan Kailasam*, Asifkhan Shanavas*, ACS Applied Bio Materials, 2022 (https://doi.org/10.1021/acsabm.1c01219)

Photodynamic therapy with CNQDs